Abstract
Madin-Darby Bovine Kidney (MDBK) cells were permanently infected with a virus following inoculation and long-term incubation with lymphatic tissue from a 7-year-old bovine with lymphocytic type of malignant lymphoma. The virus, passed to a calf by dripping a suspension into its eyes, nostrils, and mouth, was recovered from lymphatic tissue 106 days later. One of 6 calves inoculated with a quantity of concentrated virus suspension developed persistent lymphocytosis in approximately 2 years. Permanently infected MDBK cells of a line infected for several months were agglutinated by both concanavalin A and wheat germ lipase contaminant. Electron micrographs of thin sections of cells revealed many virions in the nuclei. Enveloped particles ranged from 47 to 58 mμ in diameter. Autoradiographic studies resulted in labelling the intranuclear virions of infected cells with uridine-5-3H. Incorporation of isotope was not inhibited by FUdR and actinomycin D.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
