Abstract
A Site of Action of Phenethylbiguanide, a Hypoglycemic Compound
Highlights
Since the results from the above experiments suggested a metabolic block in the citric acid cycle and in the area involving the oxidation of succinatc, we examined the effect of DBI on the succinic oxidase and cytochrome oxidase enzyme systems
It is apparent that this hypoglycemic agent, when tested at 0.5 mg. per ml. (0.002 M), substantially depresses the oxidation of glucose, acetate, and succinatc but has a much less intense inhibitory effect on the oxidation of cit,rate and fumarate
I Cytochrome c a oxidase that the most sensitive site of inhibition is at a point between succinate and cytochrome c. This conclusion is not in complete agreement with that recently reported by Steiner and Williams (6). The observations of these authors suggest that the most likely site of inhibition is on cytochrome oxidase
Summary
Procedure Used with Adipose Tissue-Nonfasted male rats (Sprague-Dawley) weighing approximately 200 gm. were decapitated and exsanguinated. Procedure Used with Adipose Tissue-Nonfasted male rats (Sprague-Dawley) weighing approximately 200 gm. The pair of epididymal adipose tissues were quickly removed, trimmed of blood vessels, weighed, and deposited in flasks containing the incubation medium. Tissue weight ranged from 200 to 400 mg. Paired tissues of equal weight from a given rat were used as a set, one the control tissue for the other. Erlenmeyer flasks with a center well) contained 3 ml
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