A single high-cholesterol, high-fat meal preferentially increases low molecular weight apolipoprotein B concentration in rat plasma.

J G Delamatre,B R Krause,L Wong,C A Hoffmeier,P S Roheim

doi:10.1016/s0022-2275(20)34294-2

Abstract

Rats were fed either rat chow (control), chow + 20% olive oil (olive oil), or chow + 20% olive oil + 2% cholesterol (olive oil/cholesterol) as a single meal to study the short-term effects of fat and the above combination of fat/cholesterol-containing diets on plasma apoB concentration and its influence on the distribution of apoB subspecies. Rats were given their meals and allowed to consume them ad libitum until they were killed, 3 hr or 9 hr afterwards. Three hours after feeding, serum triglyceride concentrations were increased to the same extent in both the olive oil and olive oil/cholesterol-fed rats as compared with concentrations in control rats, but serum apoB concentrations did not differ among the groups. Nine hours after feeding, serum triglyceride concentrations were still equally elevated in both experimental groups; however, in the olive oil/cholesterol-fed rats, total serum apoB as well as total serum cholesterol were increased above both the control and olive oil groups. In addition, the d less than 1.21 g/ml lipoprotein apoBl/apoBh ratio of the olive oil/cholesterol-fed rats was greatly increased at 9 hr, whereas apoBl/apoBh ratio in the d less than 1.21 g/ml fraction of the olive oil group was unchanged, despite the increase in plasma triglyceride concentration. In the olive oil/cholesterol-fed rats at 9 hr, cholesterol, total apoB, apoBl, and apoBh of both VLDL and IDL were greater than in the control or olive oil rats. In d less than 1.21 g/ml lipoproteins, VLDL, and IDL, the increases in apoBl concentrations were of a greater magnitude than the increases in apoBh.(ABSTRACT TRUNCATED AT 250 WORDS)

Highlights

L as a single meal to study the short-term effects of fat and the above combination of fat/cholesterol-containing diets on plasma apoB concentration and its influence on the distribution of apoB subspecies
ApOBh ratio in the olive oil/cholesterol-fed rats was not increased at 3 hr, intermediate density lipoproteins (IDL) apoB1 concentration was higher than in either the control or olive oil groups; since this represented only a small fraction of the total apoB, it was not reflected in the d < 1.21 g/ml lipoprotein values.WThese observations indicate that dietary cholesterol affects apoB1 metabolism differently than apoBh metabolism. -DeLamatre, J
Serum triglyceride concentration indicated chylomicron production was taking place at 3 hr, this did not result in a detectable change in serum apoB concentrations

Summary

Introduction

L (olive oil/cholesterol) as a single meal to study the short-term effects of fat and the above combination of fat/cholesterol-containing diets on plasma apoB concentration and its influence on the distribution of apoB subspecies. Rats were given their meals and allowed to consume them ad libitum until they were killed, 3 hr or 9 hr afterwards. ApOBh ratio in the olive oil/cholesterol-fed rats was not increased at 3 hr, IDL apoB1 concentration was higher than in either the control or olive oil groups; since this represented only a small fraction of the total apoB, it was not reflected in the d < 1.21 g/ml lipoprotein values.WThese observations indicate that dietary cholesterol affects apoB1 metabolism differently than apoBh metabolism.

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Results

Conclusion