Abstract
Tomato yellow leaf curl virus (TYLCV), a member of the Geminivirus genus, has caused an epidemic disease in tomato worldwide, including in Azerbaijan. TYLCV is a phloem-limited virus that is transmitted in a semi-persistent manner by whiteflies (Bemisia tabaci). Experimental propagation of TYCV has been performed primarily by using the single-leaflet grafting inoculation method. To develop a simple and effective method for transmission of TYLCV, we investigated grafting single-leaflets from tomato plants infected with TYLCV to recipient tomato seedlings. Fifty-one (51) tomato seedlings were tested and forty-eight (48) were grafted successfully with single-leaflets infected with TYLCV. Among them, forty-six (46) seedlings (92%) were systemically infected with TYLCV and developed typical symptoms. In our study, we found that single-leaflet grafting could provide a sufficient amount of inoculum for the transmission of TYLCV to the grafted tomato seedlings. The reported results regarding the development of the TYLCV inoculation and monitoring method and its application to study host plant-virus interaction showed that it could be used as an efficient alternative inoculation method.
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