Abstract
A simplified method is proposed for the rapid determination of vitamin A in animal tissues in the presence of a large amount of β-carotene, using a modified method for dehydration of vitamin A. Special care for the exclusion of moisture was not required, since the dehydration reagent used was alcoholic hydrochloric acid containing a small amount of water. Evaporation process was not needed, because the dehydration reaction was carried out in a benzene solution and the sample solution was not diluted with the reagent. Single extraction with benzene was enough to extract vitamin A and β-carotene almost completely from the digested homogenate. The recoveries of vitamin A and β-carotene added to the tissue homogenates prior to the alkali digestion were satisfactory for ordinary biochemical researches. Application of this method to the semimicro determination of vitamin A is also presented. The determination of the amounts of vitamin A and carotene in about 10 samples were possible in about 6 hours.
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