Abstract
A simplified economical method for assaying acetylcholinesterase inhibitors was devised. A flesh fly, Sarcophaga peregrina Robineau-Desvoidy, cell line, NIH-SaPe-4, was cultured in 96-well microplates at 25°C. After 2 days culture, the culture media was removed and phosphate buffer was introduced. The cells were disintegrated by freezing–thawing, and a reaction mixture containing substrate, inhibitor, and colour developer was added. The change of absorbance at 405 nm at 25°C was measured with a microplate photometer and the concentration of the test substance required to inhibit the enzyme reaction by 50% (I50) was calculated.
Full Text
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call