Abstract
A two-stage, thin-layer chromatographic technique for the unequivocal separation of phosphatidylglycerol (PG) from other leaf glycerolipids is described. The method takes advantage of the partitioning of PG into petroleum ether from acidified aquous methanol and of its high chromatographic mobility on acidified adsorbents. Although particularly suited to the analyses of fatty acids in PGs from diverse leaves, the technique may also be used preparatively. The high content of saturated fatty acids within the PG of C4, dicotyledenous plants but not of C4 grasses is confirmed. Hexadeca- trans-3-enoic acid was absent from the leaf PG of all of the orchid species analysed.
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