Abstract
Trigonella foenum-graecum L. is commonly known as fenugreek. It is an annual herb, commonly used in many parts of India. In the present study total flavonoid estimation, antioxidant activity and isolation of quercetin were carried out. Sequential extraction was performed using chloroform, ethyl acetate and methanol. Highest yield was observed in methanol extract (10.04%). Among the three solvents ethyl acetate (3.2mg/g) showed maximum amount of total flavonoid content followed by chloroform (3.05mg/g) and methanol (1.0mg/g) extracts. Antioxidant potential was evaluated by DPPH free radical scavenging assay and Super oxide scavenging assay for ethyl acetate and chloroform extracts. Among the extracts ethyl acetate extract showed maximum antioxidant potential at 67.45% and 48.565% for DPPH free radical scavenging assay and Super oxide scavenging assay respectively. The isolation of quercetin was performed in ethyl acetate extract and validated using HPLC by comparing with known Standard quercetin.
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