Abstract
A simple assay for the measurement of renin concentration and renin substrate concentration in microliter quantities of rat plasma is described. In the plasma renin concentration assay 5 μl of rat plasma was incubated for 2 hr at 37°C in the presence of 20 μl renin substrate (plasma from nephrectomized rats) and 5 μl inhibitor-buffer solution pH 7.4. At the end of the enzymic incubation the generated angiotensin I was estimated by radioimmunoassay. Angiotensin I antisera and iodinated angiotensin I were added to the same tube and left to equilibrate at 4°C for 15 hr. EDTA, dimercaprol, and 8-hydroxyquinoline were used to inhibit converting enzymes and arigiotensinases. Reproducibility of the assay was good, and recovery of added angiotensin I complete. The normal range of plasma renin in rats was between 7 and 15 pmol angiotensin 1/ml/hr.
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