Abstract
The well known method of direct tissue printing onto nitrocellulose filters has been adapted for the detection of the spatial distribution of leaf proteins. Efficient transfer of proteins requires abrasion of the epidermis and application of high pressure. The transferred proteins can be recognized by antibodies, and differential accumulation of tobacco ( Nicotina tabacum L.) pathogenesis-related proteins of class 1 could be visualised after infection with tobacco mosaic virus, in contrast to the uniform distribution of ribulose-1,5-bisphosphate carboxylase-oxygenase. Since the technique is simple and requires little time, it is well suitable for the analysis of a large number of samples.
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