A simple method to fabricate a Prussian Blue nanoparticles/carbon nanotubes/poly(1,2-diaminobenzene) based glucose biosensor

Abstract

A novel Prussian Blue nanoparticles/carbon nanotubes/poly(1,2-diaminobenzene) based glucose biosensor was fabricated by a simple and fast method. Firstly, the Prussian Blue (PB) nanoparticles were direct electrodeposited onto the surface of a glassy carbon electrode modified with multiwall carbon nanotubes (MWNTs) in a short time. Then an ultrathin conducting poly(1,2-diaminobenzene) film was electrodeposited onto the surface of PB/MWNTs nanocomposite to immobilize the glucose oxidase via glutaraldehyde cross-linking. The experiments results showed that the stability of the PB nanoparticles was greatly improved by the MWNTs and the PB/MWNTs nanocomposite exhibited an excellent synergistic electrocatalytic effect toward reduction of hydrogen peroxide. The biosensor showed excellent performances toward determination of glucose. The linear range of the glucose biosensor is from 10 µM to 2.5 mM, with a detection limit of about 5 µM. The response is within less than 5 sec.