Year-end Sale % OFF 
Abstract
A simple method is described for the preparation from sheep pituitaries of ACTH of 100 U.S.P. units/mg. with a yield of 50 mg./kg. wet weight of glands. The method Involves aqueous extraction of ground sheep pituitaries with 0.1N HC1, removal of coagulated proteins at 85° C and pH 5.6, oxycellulose adsorption of the hormone from the supernatant and elution with 0.1N HC1, followed by column chromatography on IRC-50. The oxycellulose eluate and the IRC-50 column eluate are both dialyzed. The ACTH is characterized in hypophysectomized rats by its ability to increase the adrenal weights and repair the morphology, decrease the thymus weights, deplete adrenal ascorbic acid, and stimulate calorigenesis and erythropoiesis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
