Abstract
A simple plaque method for detecting cells producing antibodies of specific immunoglobulin class in the chicken is described. This method is based upon the inability of chicken antibody to activate guinea-pig complement. IgM- and IgG-specific plaque-forming cells in the spleen of chickens immunized with sheep red blood cells were detected using guinea-pig complement and rabbit anti-chicken-μ- or γ-chain serum. The specificity of the immunoglobulin class of plaques was confirmed by the abolition of class-specific hemolytic plaques after treatment of the lymphoid cells with rabbit antisera specific for chicken heavy chains in the presence of guinea-pig complement. The method is very simple and rapid, and IgM and IgG specific plaques are reliably detected.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call