A Simple and Selective Fluorescent Sensor Chip for Indole-3-Butyric Acid in Mung Bean Sprouts Based on Molecularly Imprinted Polymer Coatings.

Jiahua Chang,Bota Bahethan,Burabiye Yakup,Mamatimin Abbas,Turghun Muhammad

doi:10.3390/s17091954

Abstract

In this paper, we report the preparation of molecularly imprinted polymer coatings on quartz chips for selective solid-phase microextraction and fluorescence sensing of the auxin, indole-3-butyric acid. The multiple copolymerization method was used to prepare polymer coatings on silylated quartz chips. The polymer preparation conditions (e.g., the solvent, monomer, and cross-linker) were investigated systemically to enhance the binding performance of the imprinted coatings. Direct solid-phase fluorescence measurements on the chips facilitated monitoring changes in coating performance. The average binding capacity of an imprinted polymer coated chip was approximately 152.9 µg, which was higher than that of a non-imprinted polymer coated chip (60.8 µg); the imprinted coatings showed the highest binding to IBA among the structural analogues, indicating that the coatings possess high selectivity toward the template molecule. The developed method was used for the determination of the auxin in mung bean extraction, and the recovery was found to be in the range of 91.5% to 97.5%, with an RSD (n = 3) of less than 7.4%. Thus, the present study provides a simple method for fabricating a fluorescent sensor chip for selective analysis.

Highlights

Phytohormones, which are typically present at low concentrations in plant tissues, regulate the growth and development of plants [1]
The polymer performance was greatly dependent on the solvent, functional monomer, and cross-linking monomer used in the polymerization and the adsorption solvent, and these parameters were each optimized in the study
To realize automatic analysis and to minimize the sample volume required for measurement, the construction of an on-line detection apparatus using the chip as a flow cell is in progress

Summary

Introduction

Phytohormones, which are typically present at low concentrations in plant tissues, regulate the growth and development of plants [1]. An important group of phytohormones, were the first of the major plant hormones to be discovered. These hormones, including indole-3-acetic acid (IAA), indole-3-propionic acid (IPA), and indole-3-butyric acid (IBA), are involved in many aspects of plant growth and development [2]. The second auxin, IBA, was discovered to promote rooting and was determined to be even more effective than IAA [3,4]. Due to the higher stability of IBA versus that of IAA, both in solution and in plant tissue, IBA has a greater ability to promote adventitious root formation than does IAA

Methods

Results

Conclusion