Abstract

A procedure has been developed in which fibroblasts are harvested from guinea-pig kidney and grown to confluency. Assay tubes containing graded concentrations of substances under examination are inoculated with portions of these fibroblasts and DNA synthesis is measured by means of [ 3H]thymidine uptake. Cytotoxicity is observed as an inhibition of DNA synthesis. This assay procedure was shown to be sensitive to three different cytotoxic agents, namely hydroxyurea, which is a metabolic inhibitor, sodium lauryl sulphate, a compound which lyses cells, and Rose Bengal, a phototoxic agent. It could be used to investigate the effect of a wide range of compounds that might come into contact with living cells.

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