Action de l'acroléine sur les synthèses d'acides nucléiques in vivo

Abstract

Summary The present work intends to study the action of acrolein on hepatic and pulmonary nucleic acid metabolism of partly hepatectomized rats. Quantity and action time of acrolein on nuclear and nucleolar nucleic acids turnover have been successively considered. At the hepatic level, the variations of DNA and RNA synthesis are parallel and are proportional to the quantity of acrolein injected. In the lung, the inhibition of DNA synthesis is proportional to the dose of acrolein injected, (between 50 an 270 μg for 100 g of rat). When acrolein acts during variable periods of time (30 mn to 6 hours), the inhibition of hepatic nuclear DNA synthesis occurs very early (30 mn), and increases with time. The inhibition of RNA synthesis occurs after a longer time (2 hours). In the lungs, the results are similar though acrolein has no effect on DNA synthesis during one hour following its injection. Acrolein does not seem to have a specific action on the system of ribosomal RNA precursors as inhibition of synthesis is similar if we study either nuclear or nucleolar hepatic nucleic acid turnover.