Abstract
A simple and rapid sample preparation method was developed and validated for multi-class analysis of veterinary drug residues in bovine kidney and liver. Sample preparation procedure was performed using acetonitrile and trichloroacetic acid for protein precipitation followed by ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) analysis. The proposed method was validated according to the criteria of the Commission Decision 2002/657/EC evaluating linearity, selectivity, accuracy and precision, determination limit (CCα), and detection capacity (CCβ). Linearity presented r 2 ≥ 0.99 for all the target compounds, and recoveries ranged from 80 to 110 % with RSD ≤17 % for intra- and inter-day assay. Values of CCα and CCβ ranged from 11 to 1096 and from 12 to 1191 μg kg−1, respectively. The proposed sample preparation followed by UHPLC-MS/MS analysis was suitable for the determination of 20 veterinary drug residues in bovine kidney and liver in routine analysis. Method applicability was evaluated using commercial samples.
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