Abstract
We report on the synthesis of dopamine loaded magnetic nanoparticles (MNPs) for new, simple, fast and repeatable extraction of ochratoxin A from different solvents and milk without utilizing immunoaffinity columns and even high-tech devices. To this end, Fe 3O4 nanoparticles (NPs) were synthesized using thermal decomposition reaction and dopamine (DPA) was then conjugated with Fe3O4 nanoparticles (NPs) to form Fe3O4-DPA NPs. Dynamic light scattering, field emission scanning electron microscopy and transmission electron microscopy revealed an average size of about 15 nm for Fe3O4-DPA NPs. Moreover, zeta potential measurement and vibrating sample magnetometer confirmed positively charged (16.8 mV) and superparamagnetic behavior of MNPs, which are effective factors for a good adsorbent in the extraction. Various solvents and different effective parameters were measured until acetonitrile:methanol was selected as the best extraction solvent. In addition, based on the pH-partition theory, with changes in pH, we were able to increase and enhance the extraction rate to 94%. Moreover, the ability of Fe3O4-DPA NPs in solid phase extraction of ochratoxin A from spiked milk was evaluated. The recovery rate for extraction of OTA from milk was 68%.
Highlights
A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin AAzam Sargazi,a Amin Aliabadi,a,b Ali Rahdari,a,b Samin Allahdini-Hesaroiyeh,a,b Massoud Nejati-Yazdinejad c and Mostafa Heidari Majd*,a aFaculty of Pharmacy and bStudent Research Committee, Zabol University of Medical Sciences, Zabol, Iran cDepartment of Chemistry, Faculty of Sciences, University of Zabol, Zabol, Iran
Mycotoxins are secondary fungal metabolites that can contaminate agricultural commodities and animal feeds.[1]
The synthesis of Fe3O4-dopamine hydrobromide (DPA) NPs was initiated through the synthesis of Fe3O4, the core of NPs, at 270 °C by thermal decomposition reaction of Fe(acac)[3] in the presence of oleylamine as a reducing, capping, and monodisperse agent.[23,24,25]
Summary
Azam Sargazi,a Amin Aliabadi,a,b Ali Rahdari,a,b Samin Allahdini-Hesaroiyeh,a,b Massoud Nejati-Yazdinejad c and Mostafa Heidari Majd*,a aFaculty of Pharmacy and bStudent Research Committee, Zabol University of Medical Sciences, Zabol, Iran cDepartment of Chemistry, Faculty of Sciences, University of Zabol, Zabol, Iran. We report on the synthesis of dopamine loaded magnetic nanoparticles (MNPs) for new, simple, fast and repeatable extraction of ochratoxin A from different solvents and milk without utilizing immunoaffinity columns and even high-tech devices. To this end, Fe3O4 nanoparticles (NPs) were synthesized using thermal decomposition reaction and dopamine (DPA) was conjugated with Fe3O4 nanoparticles (NPs) to form Fe3O4-DPA NPs. Dynamic light scattering, field emission scanning electron microscopy and transmission electron microscopy revealed an average size of about 15 nm for Fe3O4-DPA NPs. zeta potential measurement and vibrating sample magnetometer confirmed positively charged (16.8 mV) and superparamagnetic behavior of MNPs, which are effective factors for a good adsorbent in the extraction.
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