Abstract
Abstract A simple and economical procedure has been set up for the liquid scintillation counting of both soluble (aqueous solutions of pure tritiated thymidine) and insoluble (tritiated culture pellets of stimulated or unstimulated lymphocytes) compounds on glass-fibre filter discs. It has been found that the radioactivity of both the soluble and insoluble compounds can be detected in only 0.1 ml of scintillation cocktail (aqueous counting scintillant, ACS). The addition of larger amounts of scintillator (0.1–3 ml) to vials containing insoluble compounds in no way increases the counting rate. With soluble compounds (previously dried at 100°C), increased quantities of scintillator (0.1–1 ml) yield slightly higher counts, but with volumes of 2 or 3 ml the counts remain stable.
Published Version
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