A simple and accurate PCR method for detection of genetically modified rice

Payam Safaei,Gholamreza Jahed Khaniki,Ebrahim Molaee Aghaee,Sassan Rezaie,Setareh Agha Kuchak Afshari

doi:10.1007/s40201-019-00401-x

Abstract

BackgroundLegislation regulating for labeling and use of genetically modified (GM) crops are increased considerably worldwide in order to health and safety assurance of consumers. For this purpose, a polymerase chain reaction (PCR) method has been developed for detection of GM rice in people’s food diet.MethodsIn this study, eighty-one non-labeled rice samples were collected randomly from different market sites of Tehran, Iran. In order to analysis, rice genomic DNA was extracted using MBST DNA extraction kit and subsequently, sucrose phosphate synthase (SPS) gene was used to confirm the quality of extracted DNA. Then, cauliflower mosaic virus (CaMV) 35S promoter and Agrobacterium nopaline synthase (NOS) terminator were selected as screening targets for detection of GM rice sequences by PCR.ResultsAccording to our results, 2 out of 81 (2.4%) samples tested were positive for CaMV 35S promoter while no positive result was detected for NOS terminator.ConclusionThe obtained data indicated that this method is capable to identify the GM rice varieties. Furthermore, it can demonstrate the possibility of the presence of GM rice in Tehran’s market, thus putting emphasis on the requirement for developing a precise approach to evaluate this product.

Highlights

Legislation regulating for labeling and use of genetically modified (GM) crops are increased considerably worldwide in order to health and safety assurance of consumers
Genomic DNA was extracted from all samples using the DNA Extraction kit from Plant Materials (MBST, IRAN) according to the instruction, some adjustments were used to improve the quality of DNA
Arun et al (2013) screened maize and soy in processed foods for detection of cauliflower mosaic virus (CaMV) 35S promoter and nos terminator by polymerase chain reaction (PCR) technique, the results indicated that 25 of the 100 (25%) samples were GM positive [20]

Summary

Introduction

Legislation regulating for labeling and use of genetically modified (GM) crops are increased considerably worldwide in order to health and safety assurance of consumers. For this purpose, a polymerase chain reaction (PCR) method has been developed for detection of GM rice in people’s food diet. J Environ Health Sci Engineer (2019) 17:847–851 gene into food materials for humans has not been systematically shown in the literature. Their detection and labelling is required for increasing the consumer awareness [10]. In compared to other methods such as the enzyme-linked immunosorbent assay (ELISA), PCR has higher specificity to acquire reliable results [14]

Methods

Results

Conclusion