Abstract
Herein, a Bi <sub>2</sub>MoO <sub>6</sub> nanosheet (BMONS)‐based sensing platform for signal‐on photoelectrochemical (PEC) detection of carcinoembryonic antigen (CEA) was successfully constructed by employing an enzyme‐catalyzed reaction as the signal amplification method. Specifically, as the CEA concentration escalates, a substantial accumulation of immunological complexes was triggered by the interaction between antigens and antibodies, which promptly concentrate glucose oxidase (GOD) within the wells of a 96‐well microplate. These sequestered GOD molecules subsequently catalyze the hydrolysis of glucose to yield H <sub>2</sub>O <sub>2</sub>, an efficient hole scavenger, leading to significant amplification of the PEC response from the photosensitive materials. Under meticulously optimized conditions, a broad linear detection range spanning from 0.05 to 50 ng mL <sup>–1</sup> with a low limit of detection (LOD) of 0.023 ng mL <sup>–1</sup> has been realized for the detection of CEA. This exceptional performance underscores the successful implementation and efficacy of our BMONS‐based PEC sensing platform, which offers promising avenues for sensitive and selective biomarker detection.
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