Abstract
Illumina's Nextera Tn5 transposase-based tagging-and-fragmentation (“tagmentation”) workflow has become one of the most widely used next-generation DNA-sequencing (NGS) protocols. However, enzymatic fragmentation of DNA, including transposase-mediated insertion reactions, is never completely free of sequence specificity. Such intrinsic sequence-fragmentation biases can distort apparent locus copy numbers in the final assembled genome, potentially in combination with additional sequence-dependent effects arising in DNA-amplification steps.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
