Abstract
Growth of influenza virus type A2, strain Japan/305/57 (formerly grown in the allantoic cavity of 11-day old chick embryos), was obtained in cultures of chick embryo fibroblasts. Virus, passed four times in chick embryo fibroblasts, followed by one passage in the allantoic sac of 11-day old embryonated eggs, gave clear plaques in a chick embryo monolayer under a serum-free agar overlay with an added DEAE-dextran concentration of 0.5 mg./ml. After three days incubation at 37° C in stoppered flasks the plaques reached a diameter of 2–3 mm. An approximately straight-line dose-response relationship was observed between the square root of the number of plaques produced and the relative concentration of virus. Specificity was demonstrated by quantitative neutralization with immune serum.
Published Version
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