Abstract
A rapid immunochromatographic assay (ICA) was developed for the detection of semicarbazide (SEM), a nitrofurazone metabolite in meat specimens. Colloidal gold–labeled monoclonal antibody specific to a SEM derivative, 4[(4-carboxyphenyl)methylene]-hydrazinecarboxamide (CPSEM), was used as a probe for the presence of SEM. The assay is based upon the competitive reactivity theory that SEM derivatized with 4-carboxybenzaldehyde (4-CBA) competes with CPSEM-conjugated bovine serum albumin for binding the colloidal gold–labeled monoclonal antibody, the result of which could be read directly by naked eyes. Under optimal conditions, the visual detection limit is 0.72 ng mL−1, which demonstrated the high sensitivity of this assay. The stability test indicated that the immunochromatographic strips could be used within 7 weeks at room temperature without significant loss of activity. Spiked pork samples were detected by ICA and confirmed by enzyme-linked immunosorbent assay (ELISA). The assay time for SEM detection was within 5 min, suitable for a rapid on-site testing for meat samples.
Published Version
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