Abstract
Summary A sensitive high-performance thin-layer chromatographic (HPTLC) method has been developed for estimation of amentoflavone, an important bioactive biflavonoid found in many medicinal plants. Amentoflavone was isolated from Biophytum sensitivum, characterized, and used as reference standard. The HPTLC method was validated for precision (intra- and inter-day), repeatability, and accuracy. The method was found to be precise – intraday and inter-day RSD, for different concentrations, were 0.52–1.36% and 1.87–3.41%, respectively. Instrumental precision and repeatability of the method were found to be 0.098 and 2.1 (%CV), respectively. The accuracy of the method was checked by performing a recovery study at two levels; average recovery was found to be 98.86%. The method was used for the estimation of amentoflavone from B. sensitivum and Putranjiva roxburghii. The method was found to be accurate and precise and could be used for the estimation of amentoflavone from plant materials.
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