Abstract
A precise and very efficient tandem mass spectrometry method (LC-MS/MS) was developed to quantify the resveratrol in the peanut matrix. Two mobile phases made up of water and acetonitrile in a ratio of 98:2 (%v/v) with 0.1% formic acid and acetonitrile and water in a percentage of 98:2 %(v/v) with 0.1% formic acid were used in the LC-MS/MS analysis of resveratrol on a BEH C-18, 130A° (50mm 2.1mm, 1.7μm) analytical column with 0.3 mL/min of flow rate. A triple quadrupole mass spectrometer and a positive electrospray ionization system were used to quantify resveratrol in multiple reaction monitoring (MRM) modes. The technique was evaluated and was found capable of quantifying resveratrol in peanut samples starting at 10 ng/g in compliance with SANTE guidelines.
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