Abstract
We report a voltammetric aptasensor based on the graphite nanocrystals in a bed of tetrahedral amorphous carbon (GNC-TAC) and guanine (G)-rich aptamers alkylated with melphalan anticancer for determination of ultratrace Pb2+ in the clinical samples. In this work, an adhesive and stable layer of GNC-TAC was formed on the cellulose paper by the ion beam sputtering deposition (IBSD) method. This porous conductive nanographite film plays a key role in providing a large area for adsorption of ferrocene carboxylic acid (FC-COOH) anticancer as a redox indicator. Then, melphalan that attaches the alkyl groups to the guanine bases of DNA aptamer was bonded covalently to the carboxylic groups of FC-COOH via the aid of EDC/NHS. The introduction of Pb2+ ions to the aptamer/melphalan/FC-COOH/GNC-TAC/paper electrode leads to the formation of a G-quadruplex (G4)/Pb2+ complex and dissociation of this complex from the modified electrode surface. The target-induced aptamer release from melphalan/FC-COOH/GNC-TAC/paper electrode makes the sensing interface smaller which results in an increase in the current signal. Based on this signal-on mechanism, the sensitive detection of Pb2+ ions in the dynamic linear range (DLR) of 0.5–700.0 μg L−1 is realized (limit of detection (LOD): 0.15 μg L−1). The present strategy shows remarkable advantages including high selectivity and wide detectable range. More importantly, the good consistency of the results obtained with the aptasensor and those by the quality control material (QCM) analysis and atomic absorption spectroscopy equipped with graphite furnace (GF-AAS) technique showed that the proposed strategy has a high potential for Pb2+ detection in the clinical samples.
Published Version
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