Abstract
We describe a semi-automatic, computerized method of quantitative densitometry for regional analysis of whole brain autoradiographs. The technique features menu-driven software and verbal prompting for data via a speech synthesizer. Advantages over manual methods include an 80% reduction in the time required for data collection, easy retrieval and statistical analysis of data, and the production of a large data base for future use. The application of this system to the [ 14C]2-deoxy- d-glucose autoradiographic method for measurement of local cerebral glucose utilization is described. This system makes available computerized densitometry at a modest cost.
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