A selective and sensitive fluorescent sensor for cysteine detection based on bi-8-carboxamidoquinoline derivative and Cu2+ complex.

Abstract

In this paper, a novel fluorescent sensor 1 for selective and sensitive detection of cysteine was developed based on a complex between bi-8-carboxamidoquinoline derivative ligand (L) and Cu2+ . The interaction of Cu2+ with the ligand causes a dramatic fluorescence quenching most likely due to its high affinity towards Cu2+ and a ligand-metal charge transfer (LMCT) process. The in situ generated L-Cu2 complex was utilized as a chemosensing ensemble for cysteine. In the presence of cysteine, the fluorophore, L, was released from L-Cu2 complex because of the strong affinity of cysteine to Cu2+ via the Cu-S bond, leading to the fluorescence recovery of the ligand. The proposed displacement mechanism was confirmed by the results of mass spectrometry (MS) study. Under optimized conditions, the recovered fluorescence intensity is linear with cysteine concentrations in the range 1×10-6 mol/l to 8×10-6 mol/l. The detection limit for cysteine is 1.92×10-7 mol/l. Furthermore, the established method showed a highly sensitive and selective response to cysteine among the 20 fundamental α-amino acids used as the building blocks of proteins, after Ni2+ was used as a masking agent to eliminate the interference of His. The proposed sensor is applicable in monitoring cysteine in practical samples with good recovery rate.