Abstract
The ability of a previously developed sandwich-type enzyme-linked immunosorbent assay (ELISA) for discriminating incorrectly folded recombinant human interferon α-2b (IFN-α2b) molecular species from multi disulphide-bonded species was investigated. This ELISA was applied to evaluate and improve the effectiveness of the renaturation of IFN-α2b, a step that is currently used in the large-scale production of IFN-α2b produced in recombinant Escherichia coli strains.
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