Abstract
We proposed a new saccharides sensor developed by symmetrical optical waveguide (SOW)-based surface plasmon resonance (SPR). This unique MgF 2/ Au / MgF 2/Analyte film structure results in longer surface plasmon wave (SPW) propagation lengths and depths, leading to an increment of resolution. In this paper, we managed to decorate the dielectric interface ( MgF 2 layer) by depositing a thin polydopamine film as surface-adherent that provides a platform for secondary reactions with the probe molecule. 3-Aminophenylboronic acid (3-PBA) is chosen to be the saccharides sense probe molecule in the present work. The aqueous humor of Diabetes and Cataract patient whose blood glucose level is normal are analyzed and the results demonstrated that this sensor shows great potential in monitoring the blood sugar and can be adapted in the field of biological monitoring in the future.
Highlights
Saccharides are widespread in nature and play an essential role in most the biological processes, such as cell-to-cell interactions, biological recognition,[1] and in nutrition, metabolism, cell structure formation and immunological protection.[2,3,4] the detection of sugar at high levels of sensitivity and selectivity is very important in medicine, the food industry and biochemical science.[5]
We proposed a saccharide sensor developed by symmetrical optical waveguide (SOW)-based surface plasmon resonance (SPR)
As the samples °owed upon the surface of sensor chips, this intensity change of the re°ected light was detected to demonstrate the refractive index change of analyte which directly correlated the e®ects of the biochemical reactions on the sensor surface.[18]
Summary
Saccharides are widespread in nature and play an essential role in most the biological processes, such as cell-to-cell interactions, biological recognition,[1] and in nutrition, metabolism, cell structure formation and immunological protection.[2,3,4] the detection of sugar at high levels of sensitivity and selectivity is very important in medicine, the food industry and biochemical science.[5]. PBA and its derivatives have a boronic acid group that has been known to form covalently bonded complexes with the 1, 2- or 1, 3-diol of sugars.[5] Their formation is fast and reversible in aqueous media,[20] and they are small and °exible molecules, can be incorporated as recognition motifs into larger structures, without changing the physical properties dramatically.[21] It makes the PBA derivatives represent useful compounds for developing an analytical method for saccharides In this manuscript, we proposed a saccharide sensor developed by SOW-based SPR. This modication method does not take many steps or very long time to accomplish, which is much easier than any other methods which have been reported
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