Abstract
The advent and growth of synthetic biology has demonstrated its potential as a promising avenue of research to address many societal needs. However, plant synthetic biology efforts have been hampered by a dearth of DNA part libraries, versatile transformation vectors and efficient assembly strategies. Here, we describe a versatile system (named jStack) utilizing yeast homologous recombination to efficiently assemble DNA into plant transformation vectors. We demonstrate how this method can facilitate pathway engineering of molecules of pharmaceutical interest, production of potential biofuels and shuffling of disease-resistance traits between crop species. Our approach provides a powerful alternative to conventional strategies for stacking genes and traits to address many impending environmental and agricultural challenges.
Highlights
IntroductionPlant synthetic biology efforts have been hampered by a dearth of DNA part libraries, versatile transformation vectors and efficient assembly strategies
The advent and growth of synthetic biology has demonstrated its potential as a promising avenue of research to address many societal needs
In an effort to expedite plant research and crop engineering, we have developed an efficient and affordable method for DNA assembly into a suite of plant transformation vectors leveraging in vivo yeast homologous recombination, along with a library of over a 100 publicly available DNA parts
Summary
Plant synthetic biology efforts have been hampered by a dearth of DNA part libraries, versatile transformation vectors and efficient assembly strategies. We describe a versatile system (named jStack) utilizing yeast homologous recombination to efficiently assemble DNA into plant transformation vectors. We demonstrate how this method can facilitate pathway engineering of molecules of pharmaceutical interest, production of potential biofuels and shuffling of disease-resistance traits between crop species. In an effort to expedite plant research and crop engineering, we have developed an efficient and affordable method for DNA assembly into a suite of plant transformation vectors leveraging in vivo yeast homologous recombination, along with a library of over a 100 publicly available DNA parts
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