Abstract

Planting of individual soil particles onto an agar medium, poured into Petri dishes, is a method of studying fungal distribution between microhabitats in soil. The substitution of Petri dishes with well plates reduced both the required amount of agar medium and labour. A microshovel prepared from an injection needle facilitated the handling of individual, small size, soil particles (0.25–0.50 mm). The described method was evaluated in a study of the distribution of Fusarium within two wheat field soils from As, Norway, and from Ostermarie, Denmark. Four types of organic particles were distinguished: light coloured root pieces, dark coloured root pieces, straw pieces, and miscellaneous organic pieces. The dominating species were F. culmorum, F. oxysporum and F. avenaceum . Aspects on the value of the method with regard to fungal substrate preference are discussed.

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