Abstract
The ability of cholestanol (5α-cholestan-3β-ol) to support growth of two independently derived sterol auxotrophs, FY3 and GL7, has been examined. Growth on this stanol was precluded unless minute quantities of sterol were also available. Contaminating sterol in most cholestanol preparations or excess sterol in the inoculum used in growth studies could provide the required sterol in quantities capable of sustaining growth through an entire culture cycle. Evidence is presented for multiple functions of sterols in Saccharomyces cerevisiae .
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