Abstract
This chapter presents an overview of a replicating form of λ phage DNA. It examines some of the physical properties of intracellular phage DNA from Escherichia coli that is infected with a mutant of λ phage. With this mutant (λb+cl), bacterial lysis occurs some 50 min after infection. Phage λ contains linear DNA molecules of molecular weight 31 million, which are known to replicate by a semi-conservative mechanism. In a study, radioactive tracer techniques were employed to study the intracellular phage DNA. When unlabeled bacteria are infected with P32-labeled phage, parental DNA molecules can be identified and their fate determined. After isolation of the intracellular DNA, the parental and newly synthesized DNA can be compared with each other and with DNA from purified λ phage particles.
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