Abstract
To develop a model system for silage fermentation that is simple, rapid, and repeatable by using storable plant materials, we made several experiments of simulated silage fermentation in culture tubes. In these experiments, we used milled alfalfa hay cubes (AHC) mixed with certain amounts of water and glucose (AHC medium) and a lactic acid bacterium (LAB) was cultivated together with or without a butyric acid bacterium (BAB) and a coliform bacterium (CFB) under anaerobic conditions. The pure culture of LAB in AHC medium produced lactic acid within 24 h of incubation. The mixed culture of CFB with LAB and BAB promoted butyric fermentation. Butyric acid was produced only with high temperature (more than 30°C), low dry matter content of AHC medium (less than 20%), and prevention of lowering the medium pH. Low soluble sugar content of the medium (0.35%) or smaller inoculum size of LAB (less than 106 cfu/g) than that of CFB (107 cfu/g) was effective for the prevention of lowering the medium pH. This model syst...
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