Abstract
Recombinant microbial biosensors are known to be simple, cheap, and very efficient monitoring tools for detecting various environmental pollutants in the field. However, although various recombinant microbial biosensors have been developed for aqueous-phase samples, very few are applicable to the gas phase. Here, we report a recombinant Escherichia coli biosensor that can be used to monitor polycyclic aromatic hydrocarbons (PAHs) in both gas and aqueous phases by color development. Among the PAHs, naphthalene and salicylate are often used as model compounds, since they are less toxic than other options and they are widely used in various applications. Here, recombinant E. coli cells carrying nahR (encoding the NahR regulatory protein for naphthalene degradation)::lac Z fusion genes were constructed and suspended (for aqueous measurements) or co-immobilized (for gaseous measurements) with chlorophenol red-ß-D-galactopyranoside (CPRG). Biosensing was then performed by ß-galactosidase, which hydrolyzed CPRG as a substrate, developing detectable red color with the naked eye. The system showed selective responses to salicylate and naphthalene. Importantly, its response to naphthalene was much more sensitive (about 105-fold) in the gas phase compared to the aqueous phase. Thus, this system could potentially be used for the instrument-free, color-change-based monitoring of gaseous pollutants.
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