Abstract
Describe a microcomputer-assisted single-beam scanning spectrophotometer which is able to track the time evolution of a spectrum with a time resolution of 0.1 s and a wavelength resolution of 2.5 nm. The apparatus, which is also able to perform spectrum averaging, utilises a commercial rotating grating monochromator and is small and lightweight, so that it can be fitted on the top of a microscope to perform time-resolved spectral analysis on histological specimens. The apparatus has been implemented as a programmable IEEE488 device. This feature allows the authors to use the apparatus in conjunction with a larger computing system providing experiment management as well as data storage and off-line data processing.
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