A ratiometric fluorescence nanoprobe for ultrafast imaging of hypochlorite in living cells and zebrafish

Abstract

In this paper, we report the design and preparation of a new fluorescent nanoprobe (HC-NP420/565) that could ratiometricly detect ClO− within five seconds by fluorescence imaging in an ultrafast and highly selective manner. The ratiometric response of the probe was facilely achieved by integrating 2-oxime-coumarin as a ClO− receptor, and a 1,8-naphthimide derivative as the internal reference into a Pluronic F-127 matrix. The probe showed highly sensitive and specific responsibility towards ClO− at physiological conditions with the detection limit of 105.16 μM. Notably, the probe displayed excellent ClO− ratiometric imaging performance in living cells and zebrafish larvae, demonstrating the potential application in ClO−-related chemical biology studies.