A rapid screening method for routine congener-specific analysis of polychlorinated biphenyls in human serum by high-resolution gas chromatography with mass spectrometric detection.

Abstract

A screening method using gas chromatography/mass spectrometry in the single ion monitoring (SIM) mode for the routine congener-specific analysis of polychlorinated biphenyls (PCBs) in human serum has been developed and validated. A fast procedure incorporating both liquid-liquid and solid-phase extraction was used for clean up and enrichment. Interday and intraday precision and accuracy were assessed for the 12 congeners having dioxin-like activity and PCBs #180 and #170 by analyzing spiked samples on three subsequent days and using (13)C(12)-labelled analogues as internal standards. Furthermore, the relative errors for six non-dioxin-like congeners (PCBs #28, #52, #101, #138, #153 and #180) were measured by using certified reference materials, providing good accuracy at two different concentration levels. The limits of quantification and of detection were 0.5 and 0.2 micro g/L in serum for each congener, respectively. The method allows the chromatographic separation of at least 35 congeners and can therefore be applied to the routine monitoring of the general population for both dioxin-like and non-dioxin-like PCB congeners.