A Rapid Reversed-Phase HPLC Method for Analysis of Trans-Resveratrol in PLGA Nanoparticulate Formulation

Gurinder Singh,Roopa S Pai

doi:10.1155/2014/248635

Abstract

A rapid reversed-phase high performance liquid chromatography (RP-HPLC) method was developed for the determination of trans-resveratrol (t-RVT) in PLGA nanoparticle formulation. A new formulation of t-RVT loaded PLGA nanoparticles (NPs) with potential stealth properties was prepared by nanoprecipitation method in our laboratory. The desired chromatographic separation was achieved on a Phenomenex C18 column under isocratic conditions using UV detection at 306 nm. The optimized mobile phase consisted of a mixture of methanol: 10 mM potassium dihydrogen phosphate buffer (pH 6.8): acetonitrile (63 : 30 : 7, v/v/v) at a flow rate of 1 mL/min. The linear regression analysis for the calibration curves showed a good linear correlation over the concentration range of 0.025–2.0 μg/ml, with determination coefficients, R2, exceeding 0.9997. The method was shown to be specific, precise at the intraday and interday levels, as reflected by the relative standard deviation (RSD) values, lower than 5.0%, and accurate with bias not exceeding 15% and percentage recovery was found to be in the range between 94.5 and 101.2. The limits of detection and quantification were 0.002 and 0.007 μg/ml, respectively. The method was successfully applied for the determination of t-RVT encapsulation efficiency.

Highlights

Trans-resveratrol, trans-3,4󸀠,5-trihydroxystilbene, (Figure 1) is a natural polyphenolic compound found in a variety of foods, essentially in red grapes
Small amount of t-RVT is present in the seed and core of the grapes. t-RVT is extracted from Polygonum cuspidatum, a plant found in China and Japan [4]
A high performance liquid chromatography method for the estimation of t-RVT in a nanoparticulate dosage form has been developed according to the principles of Good Laboratory Practices

Summary

Introduction

Trans-resveratrol, trans-3,4󸀠,5-trihydroxystilbene, (Figure 1) is a natural polyphenolic compound found in a variety of foods, essentially in red grapes. It is a potent antioxidant and present mainly in the skin of grapes [1,2,3]. Small amount of t-RVT is present in the seed and core of the grapes. T-RVT is extracted from Polygonum cuspidatum, a plant found in China and Japan [4]. TRVT has been suggested to possess cancer chemopreventive [5], antioxidative [6], antiplatelet, antifungal, cardioprotective [7], and HIV/AIDS properties [8]; the mechanism(s) of these proposed effects are not fully understood [9] Resveratrol is existing as both cis- and trans-isomers, ; the transisomer is the most abundant and biologically active form. tRVT has been suggested to possess cancer chemopreventive [5], antioxidative [6], antiplatelet, antifungal, cardioprotective [7], and HIV/AIDS properties [8]; the mechanism(s) of these proposed effects are not fully understood [9]

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Results

Conclusion