Abstract
A rapid method requiring only three to four hours for completion, is described for the estimation of testosterone in male plasma. This technique uses the principle of displacement of labeled testosterone from a specific binding protein by testosterone in the sample. Separation of bound and free testosterone is effected by subsequent adsorbtion to dextran-coated charcoal particles which can be separated from plasma by centrifugation. Studies with this system indicate that with physiological amounts of known C 21, C 19 and C 18 steroids present in plasma, binding of testosterone is very specific. The excellent agreement between the binding method and a much more complex and costly, established, double isotope derivative assay, indicates a high degree of precision and accuracy. This technique appears to be a practical approach to the routine estimation of testosterone in male plasma.
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