Abstract
Abstract An accurate and reproducible procedure is described for the determination of testosterone from male plasma with the use of competitive protein binding. One milliliter of plasma is required to satisfactorily measure testosterone in plasma from normal orchiectomized men. The mean recovery of testosterone in approximately 300 samples was 60 ± 10 per cent (S.D.). The mean plasma testosterone level in 50 normal men was 5.6 ± 2.3 mμg per milliliter (S.D.). In 30 patients with carcinoma of the prostate, the mean was 7.9 ± 2.2 mμg per milliliter (S.D.) which decreased to 0.6 mμg per milliliter after orchiectomy.
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