Abstract
1. 1. The 1000 g supernatant of a tissue homogenate is layered on top of a small (< 5 ml) sucrose density gradient and centrifuged for 20 min at very high centrifugal forces in a vertical rotor. 2. 2. Microsomes can be recovered rapidly in suspended form from the middle of the gradient, well separated from mitochondria and soluble (cytosolic) components. 3. 3. Applications to cockroach midgut microsomes and mosquito abdominal tissue microsomes are described, and the method is compared to the classical differential centrifugation method. 4. 4. Cytochrome P-450 monooxygenase activities can be measured on microsomes prepared from midgut tissue of 2–3 Diploptera punctata using this method.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Comparative Biochemistry and Physiology Part B: Comparative Biochemistry
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
