Abstract
Abstract The concentration of free fucose in urine has been shown to be elevated in adults with malignancy. Current analytical methods include enzymatic analysis and high pressure liquid chromatography (HPLC) with fluorescence detection. We describe a simple HPLC method using anion exchange chromatography with pulsed electrochemical detection for the determination of free fucose in urine of children with malignancy. Separation of sugars is achieved within 7 minutes. The method is linear to 200 μmol/1 fucose with a detection limit of 0.5 μmol/1 in urine. Mean recovery was 96.6% ± 11.8%. Precision was good with coefficient of variation (CV) of 4.3% (25 μmol/1), 3.7% (50 μmol/1) and 4.9% (100 μmol/1). The median fucose: creatinine ratio in healthy children was 13.6 μmol / mmol (5.5–128.5). Children with malignancy had a median fucose: creatinine ratio of 8.1 μmol/mmol (2.6–66.4) which was significantly lower than the control subjects (p=0.038). The method enables rapid determination of free fucose in urine w...
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