Abstract
Abstract An electrochemical technic for measuring carbon dioxide (CO2) content in whole blood has been devised and evaluated. The method requires a membrane-covered pH electrode for the CO2 measurements. This electrode system permits rapid determination of CO2 content in blood samples of less than 1 ml. The measurement is performed by hemolyzing and acidifying a blood sample in such a manner that the released CO2 goes into physical solution. The increase of tension caused by this physically dissolved CO2 is measured by exposing the sample to a previously calibrated electrode. While use of the technic requires some compromise with accuracy (standard deviation of replicate samples = 0.76 volume/100 ml. compared with 0.12 volume/100 ml. for the Van Slyke manometric procedure), its convenience may outweigh this consideration in certain routine applications.
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