Abstract
Isolated scutella from immature embryos of barley and wheat, commonly used as targets for genetic transformation, revealed a considerable variation in susceptibility towards bialaphos and glufosinate (phosphinothricin) among a range of cultivars investigated. Using the pH indicator chlorophenol-red, a rapid assay was established to determine within two days genotype specific basal level of tolerance based on spectrophotometric determination of colour change in the medium. A strong correlation was observed using the response of scutella incubated for 3 weeks on callus induction medium containing different amounts of bialaphos with their ability to change colour of chlorophenol-red in the medium within two days. Thus it can be concluded that the amount of bialaphos which is needed by a given genotype to achieve an absorbance of 0.5 at 575 nm, represents a tight selection condition for the appropiate genotype. The colour change of the medium caused by phosphinothricin based agents after exposure of expiants results from the interaction between acidification due to metabolic processes and rise of pH caused by ammonia accumulation due to inhibition of glutamine synthetase.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
