Abstract
We describe an efficient method for the rapid quantitative determination of the abundance of three acidic plant hormones from a single crude extract directly by LC/MS/MS. The method exploits the sensitivity of MS and uses multiple reaction monitoring and isotopically labelled samples to quantify the phytohormones abscisic acid, jasmonic acid and salicylic acid in Arabidopsis leaf tissue.
Highlights
Phytohormones play an important role in mediating host responses to various biotic and abiotic stresses such as pathogen challenge, insect herbivory, drought, cold and heat stress
While here we report characterization of this method on Arabidopsis thaliana leaves, this method is applicable to other plant species such as tomato
Seven 10 mg samples of freeze dried material were extracted after the incorporation of deuterated hormone standards and each hormone was expressed as a ratio of phytohormone to deuterated internal standard (IS)
Summary
Phytohormones play an important role in mediating host responses to various biotic and abiotic stresses such as pathogen challenge, insect herbivory, drought, cold and heat stress. Classical SA and JA responsive molecular markers indicate that these phytohormones function antagonistically, recent studies suggest that both the timing and amplitude of hormonal signals play key roles in determining the final pathological phenotype [3,4]. Emerging evidence suggests that a key strategy of plant pathogens is to modify plant hormone levels to promote pathogenicity. Tomato DC3000, delivers ~30 effector proteins into the plant cell [5]. Experimental data suggest they act with a surprising degree of redundancy to modify host signalling pathways, and one clear strategy is to suppress or modify plant hormone responses [6,7]
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