A quantitative analysis of the incidence of certain cytoplasmic structures in the ovum of the rat during cleavage.

Abstract

The volumetric density of most cytoplasmic organelles in the segmenting ovum of the rat was determined by morphometric techniques described by Weibel, at the 1-, 2-, 4-, and 8-cell stages, and at the early and late blastocyst stages. During the course of cleavage, the volumetric density of mitochondria remained practically unchanged. The volumetric density of the Golgi complex complex was too small to permit assessment of the differences between the individual stages of development. Significant changes were found in both granular and agranular endoplasmic reticulum, secondary lysosomes, multivesicular bodies, lamellar structures and lipid droplets. Granular endoplasmic reticulum was first observed as late as the 8-cell ovum stage and its volumetric density increased further in the early and late blastocysts. The relative volume of agranular endoplasmic reticulum was highest in the 1-cell ovum and decreased during the course of cleavage. The same is true for multivesicular bodies. The volumetric density of secondary lysosomes increased during cleavage, reaching the highest values in the 8-cell ovum. Lamellar structures were the most voluminous part of the cytoplasm of the segmenting ovum at all stages. Their volumetric density, however, decreased during the course of cleavage. Lipid droplets occur in very small quantities in the 1- to 4-cell ova, but at later stages their volumetric density increased. Our findings underline the importance of acquiring quantitative information about changes in cell organelle populations for assessing morphological and functional relationships during the early stages of cleavage of the ovum.