Abstract
We combine the finite-difference time-domain method with pulse response techniques in order to calculate the light scattering properties of biological cells over a range of wavelengths simultaneously. The method we describe can be used to compute the scattering patterns of cells containing multiple heterogeneous organelles, providing greater geometric flexibility than Mie theory solutions. Using a desktop computer, we calculate the scattering patterns for common homogeneous models of biological cells and also for more complex representations of cellular morphology. We find that the geometry chosen significantly impacts scattering properties, emphasizing the need for careful consideration of appropriate theoretical models of cellular scattering and for accurate microscopic determination of optical properties.
Highlights
Recent work has suggested that elastic light scattering spectroscopy can provide a valuable, non-invasive means to quantitatively probe tissue morphology
Since only sparse references to pulsed finite-difference time-domain (FDTD) are found in the electromagnetic literature, a series of simulations were run to verify that the method produced results in agreement with Mie theory for a series of non-conducting objects
FDTD data were compared to an analytical solution computed using Mie theory
Summary
Recent work has suggested that elastic light scattering spectroscopy can provide a valuable, non-invasive means to quantitatively probe tissue morphology. Zonios et al reported a method to extract effective scatterer sizes from colon tissue spectra based on a Mie theory model [5]. In order to isolate the scattering signal due to cell nuclei from diffuse background scattering, which is typically orders of magnitude larger, Backman et al [8] and Sokolov et al [9] proposed techniques based on polarized light scattering spectroscopy. These polarization-based techniques assessed size and refractive indices of cell nuclei using Mie theory models. All of the studies noted above developed models based on Mie theory which could adequately describe experimental data, offering a potential approach to extracting quantitative information from the scattering spectra
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